A New Simple Semimicro Method for Colorimetric Determination of Urea

A new simple semimicro method for colorimetric determination of urea.

A sensitive method for the colorimetric determination of urea.

A method is described for the direct colorimetric determination of urea in biological fluids. The method depends on the reaction (first described by Wheatley, 1948) between urea, diacetylmonoxime, and phenylanthranilic acid in the presence of controlled amounts of oxidant; chloride ions are included to sensitize the reaction; manganous ions stabilize the resultant colour; and phosphate enables ...

An Improved Method for the Simple and Accurate Colorimetric Determination of Urea with Ehrlich's Reagent.

An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12.5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow...

A Semimicro Modification of the Karr Urea Determination Method Using the Somogyi Serum Filtrate.

B ECAUSE of the pressing need for conserving both the patient’s blood and laboratory time, an attempt has been made to modify the Karr urea method (1) for semimicro amounts of Somogyi filtrate (2). Since this serum filtrate was already being used for glucose in our clinical laboratory, the saving of time and material which such a method would bring-if accurate enough for clinical purposes-was o...

A simple method for the determination of serum cholesterol